Views: 5 Author: Site Editor Publish Time: 2022-12-06 Origin: Site
Potentiometric Titration for Threonine API is the name of the method. Determination
The titration method is used in this method to determine the threonine content of threonine raw materials.
This method can be used to make threonine bulk drug.
The technique is guided by the following principle: After dissolving the test sample in anhydrous formic acid and glacial acetic acid, titrate with perchloric acid using the potentiometric titration method, and then correct the titration result with a blank test. Determine the threonine content using the amount of titrant used.
1. Acetic acid from glaciers as a reagent
Formic acid produced by anaerobic bacteria
3. Perchloric acid titrant (0.1 mol/L)
4. the most effective Potassium Hydrogen Phthalate
1. A perchloric acid titrant (0.1mol/L) is used to prepare samples.
Preparation: Take 750 mL of anhydrous glacial acetic acid, add 8.5 mL of perchloric acid (70-72%), shake well, and set aside for 24 hours. If the test sample is easily acetylated, the moisture content of the solution must first be determined using the moisture measurement method, and then adjusted with water and acetic anhydride to achieve a range of 0.01% to 0.2%.
To calibrate, weigh precisely 0.16 g of the benchmark potassium hydrogen phthalate, dissolve it in 20 mL of anhydrous glacial acetic acid, add 1 drop of crystal violet indicator solution, and slowly titrate to blue with this solution. The results of the titration are then corrected with a blank test. Per milliliter of perchloric acid titration solution (0.1mol/L), potassium hydrogen phthalate weighs 20.42 mg. Calculate the concentration of this solution using the amount of potassium hydrogen phthalate consumed while consuming it.
The test sample should be precisely weighed at 0.1 g before adding 3 mL of anhydrous formic acid and 50 mL of glacial acetic acid to dissolve it. The test sample should then be potentiometrically titrated with a perchloric acid titration solution (0.1 mol/L) and the result should be compared to a blank. Corrective action trial. 1mL of perchloric acid titration solution (0.1mol/L) contains 11.91mg of C4H9NO3.
It should be noted that the term "precision weighing" refers to weighing that is accurate to a thousandth of a pound. "Precise measurement" refers to volume measurement accuracy that meets the accuracy specifications specified in the national standard for volume pipettes.